Quick guide
How to use this calculator
- Choose the complete example closest to your experiment or biological scenario.
- Replace every value with measurements from one compatible protocol and unit system.
- Review the labelled outputs and limitations before using the result in further analysis.
Calculation method
Transparent biological calculation
Shannon H′ = −Σpᵢln(pᵢ); Simpson diversity = 1 − Σpᵢ²
Only the displayed inputs are used. No hidden organism profile, clinical threshold, assay calibration or reference population is inserted.
Worked example
Three complete starting datasets
A complete introductory rarefaction scenario dataset that populates every required field.
Shannon H′ = −Σpᵢln(pᵢ); Simpson diversity = 1 − Σpᵢ²
Supported inputs
Precision and limits
Protocol boundaries
Diversity indices depend on sampling design, detection and taxonomic resolution; they are not universal ecosystem-quality grades.
Model, not biological certainty
Calculated expectations and estimates do not establish mechanism, causation, diagnosis or experimental validity.
Measurement quality
Sampling, calibration, controls, replicates and accepted laboratory procedures remain essential.
Privacy
Entered sequences and measurements remain in this browser.
